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Description
Human TRPC1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with transient receptor potential canonical 1 (TRPC1) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the transient receptor potential canonical 1 (TRPC1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transient receptor potential canonical 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transient receptor potential channel 1 (TRPC1) is a protein encoded by the TRPC1 gene. TRPC1 is an ion channel located on the plasma membrane of many human and animal cell types. It is a nonspecific cation channel, meaning both sodium and calcium ions can pass through it. TRPC1 is thought to mediate calcium entry in response to depletion of endosomal calcium stores or activation of receptors linked to the phospholipase C system. In HEK293 cells, the unit current-voltage relationship of endogenous TRPC1 channels is nearly linear, with a slope conductance of approximately 17 pS. The extrapolated reversal potential of TRPC1 channels is +30 mV. TRPC1 protein is widely expressed throughout the mammalian brain, with a cortical expression pattern similar to that of TRPC4 and TRPC5. The highest TRPC1 protein densities are found in the lateral septum, an area densely expressed by TRPC4, as well as in the hippocampus and prefrontal cortex, areas densely expressed by TRPC5. TRPC1 was the first transient receptor potential channel identified in mammals. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 1543 reviews
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Product Reviews
★★★★★ 5
Solid, grippy, and they don’t roll away!
Style: 10 Pound, Pair
I bought these because I’m finally trying to build a consistent home workout routine and didn't want to drop a fortune on name-brand weights. I’ve been using this 10lb pair for about a month now for everything from overhead presses to lunges, and honestly, they’re exactly what I was looking for.
The Pros:
• That Neoprene Grip: The coating is fantastic. Even when my hands get pretty sweaty halfway through a workout, I don’t feel like I’m going to drop them. It’s a much "softer" feel than cold metal or hard plastic.
• The Hex Shape: This is a small detail that makes a huge difference. Since the ends are hexagonal, they don't roll across the floor when I set them down between sets. They also stay put if I’m using them for "renegade rows" or floor work.
• Compact Size: They aren’t bulky. I can easily shove them under my bed or in the corner of the closet when I’m done.
• No Weird Odor: I was worried they might smell like a tire factory (which happens with some rubber-coated weights), but these had almost no scent right out of the box.
The Cons:
• Handle Diameter: The handles are a little on the thicker side. I have medium-sized hands and it’s fine, but if you have very small hands, they might feel a bit chunky to wrap your fingers all the way around.
• Color Scuffs: The navy blue looks great, but if you clank them together or drop them on concrete, the coating can scuff or show dust pretty easily.
The Verdict:
If you just need a reliable set of weights for HIIT, Pilates, or general strength training, these are a no-brainer. You're basically getting gym-quality equipment for a fraction of the price. I’ll probably be back for the 15lb set in a few months!
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Reviewed in the United States on January 27, 2026
★★★★★ 5
Simple, Comfortable, and Exactly What You Expect
Style: Rack with 3 Pairs (5, 10, and 15 Pound)
These are pretty much exactly what you want from a set of dumbbells — simple, functional, and no nonsense. The neoprene coating gives them a comfortable grip and keeps them from feeling slick in your hands, especially during longer workouts or if your hands get sweaty.
The coating also helps keep them from banging up floors or making as much noise compared to bare metal weights, which is nice if you’re working out at home.
Weight accuracy seems fine for general fitness use, and they’ve worked great for things like accessory movements, shoulder work, lighter presses, rehab-style exercises, and general muscle toning. The shape also helps keep them from rolling around everywhere when set down.
They’re not fancy, and they’re not trying to be — they’re just dependable dumbbells that do what they’re supposed to do at a fair price.
If you want straightforward hand weights for home workouts without overthinking it, these are a solid buy.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Reliable, Comfortable Dumbbells I Actually Use Every Day
Style: 12 Pound, Pair, Style: 12 Pound, Pair
I’ve been using the Amazon Basics Neoprene Dumbbell Hand Weights daily, and they’ve honestly been perfect for my routine. Whether I’m doing a quick morning workout or adding resistance to a longer session, these have held up really well.
The neoprene coating makes a big difference—it provides a comfortable, non-slip grip, even during longer workouts or when my hands get a little sweaty. They’re also easy to clean and don’t have that strong rubber smell some weights can have.
I really appreciate the hex shape too. They stay put when I set them down (no rolling across the floor), which makes switching between exercises quick and frustration-free. The weight feels evenly distributed and well-balanced, so movements feel controlled and smooth.
Overall, these are simple, no-frills dumbbells that just work. If you’re looking for reliable hand weights for everyday use—whether you’re a beginner or just need something dependable for home workouts—these are a great choice.
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Great quality and perfect for home workouts!
Style: Rack with 3 Pairs (3, 5, and 8 Pound)
I’m really happy with this dumbbell set. The weights are clearly labeled (3 lb, 5 lb, and 8 lb), which makes it super convenient to switch between exercises. The neoprene coating feels comfortable in hand and provides a good grip, even during longer workouts.
The storage stand is a big plus—it keeps everything organized and saves space, which is perfect for a small home gym. The colors are also nice and make it easy to quickly grab the weight you need.
Overall, this is a great set for beginners or anyone looking to add light strength training to their routine. Highly recommend!
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Reviewed in the United States on April 13, 2026
★★★★★ 5
I’m A PT AND USING THEM IN MY CLINIC. PLS READ HONEST AND THOROUGH REVIEW.
Style: 15 Pound, Pair
I have been a PHYSICAL THERAPIST FOR 30 YEARS and I BOUGHT THESE FOR MY CLINIC. THESE WEIGHTS ARE COVERED IN A GRIPPING MATERIAL, NEOPRENE, MAKING IT COMFORTABLE and EASIER TO USE, ALLOWS YOU TO NOT LOSE YOUR GRIP, and IT IMPROVES THE SAFETY OF THE USER. I WILL BE BUYING THE 12&20 lb sets! THE VALUE FOR YOUR MONEY IS WONDERFUL!! AMAZON BASICS REALLY CAME THROUGH. It DID NOT INCLUDE AN INSTRUCTION GUIDE TO USE WEIGHTS SAFELY AND EFFECTIVELY. STELLAR FORM IS ALWAYS ESSENTIAL.
MY WEIGHTS WENT UP TO 10 POUNDS, SO I DEFINITELY NEEDED ADDITIONAL WEIGHTS for those who can SAFELY TRANSITION . I MAINLY WORK AS A MANUAL THERAPIST …USING ONLY MY HANDS, AND MY BRAIN. TO DIAGNOSE AND TREAT ACCORDINGLY.
(**ANYONE WHO HAS BEEN TO PT KNOWS THIS KIND OF ONE ON ONE TREATMENT IS UNHEARD OF**)
I IMPLEMENTED MYOFASCIAL RELEASE, TRIGGER POINT THERAPY, DEEP TISSUE MASSAGE, AND APPROPRIATE STRETCHING AND EXERCISE . ONCE PATIENTS WERE ABLE, I INITIATED THERAPEUTIC EXERCISE. I WAS GETTING SO MANY REFERRALS THAT I MOVED FROM MY SMALL SPACE AND GOT A LARGER SPACE THAT LOOKS LIKE A TYPICAL PT CLINIC. I STILL EMPLOY ONE ON ONE TREATMENT, NO JUGGLING SEVERAL PATIENTS IN AN HOUR. I HAVE HIRED HELP TO ASSIST PATIENTS WITH EXERCISE AND STRETCHING. I PURCHASED TWO ADDITIONAL SETS ITHE SET IT I DID TO FIRST CHECK THEM OUT. I ADORE THESE AND HAVE SINCE ORDERED OTHER SETS. I LOVE TO WORKOUT AND I NEEDED TO USE THESE WEIGHTS MYSELF!! THERE ARE ALSO PATIENTS OF MINE WHO WILL PROGRESS TO THESE AS WELL. I AM SUPER IMPRESSED WITH THESE WEIGHTS AND CANNOT WAIT TO RECEIVE MY TWO OTHER SETS!! YOU WILL NOT BE DISAPPOINTED.
EVEN IF YOU SWEAT, YOU ARE STILL ABLE TO HOLD ONTO THESE WEIGHTS COMFORTABLY AND SAFELY. SAFETY IS KEY, ALWAYS. I WAS EXTREMELY DELIGHTED THAT THEY ARE SOLD AS A SET BECAUSE MANY ARE SOLD SEPARATELY. THEY FIT PROPERLY ON A UNIVERSAL WEIGHT STAND, SO THEY ARE VERY EASY TO STORE. I CANT WAIT TO GET MY OTHER SETS! IT IS REALLY SAFE AND COMFORTABLE TO USE THESE WEIGHTS.
THEY DID NOT GIVE OFF AN ODD ODOR, which is A HUGE PLUS because SOME OTHER BRANDS SMELL HEAVILY OF PLASTIC OR A CHEMICAL TYPE ODOR. THERE WAS NO ODOR AT ALL. THIS IS A HUGE PLUS. I AM COMFORTABLE SAYING THAT THESE WEIGHTS WILL LAST A LONG TIME BECAUSE OF HOW WELL THEY ARE MADE. YOU CANNOT BEAT THE PRICE EITHER!!
THESE ARE ABSOLUTELY APPROPRIATE FOR PROFESSIONAL USE, AND, THEY ARE MEANT FOR PERSONAL USE AS WELL. SOMETIMES YOU JUST CANNOT GET TO THE GYM SO THIS IS WHERE THEY ARE KEY. HAND WEIGHTS HAVE THE ABILITY TO EXERCISE EACH MUSCLE OF THE TORSO AND ARMS. THERE ARE SOME EXCELLENT WEIGHT CHARTS OUT THERE TO SHOW YOU HOW TO DO THAT. IF YOU ARE GOING TO PHYSICAL THERAPY, THEN YOUR THERAPIST CAN INSTRUCT YOU AND KEEP YOU IN GOOD FORM. YOU COULD POSSIBLY HURT YOURSELF IF YOU WORKOUT INCORRECTLY. AS FAR AS THE LOWER EXTREMITIES, YOU CAN DO GROSS MOTOR EXERCISES BY HOLDING THESE WEIGHTS TO GET YOUR LEG MUSCLES BY DOING SQUATS, WALKING LUNGES, TOE RAISES, AND MORE. I DONT BELIEVE THAT MANY PEOPLE REALIZE JUST HOW MUCH YOU CAN DO FULL BODY EXERCISES WITH JUST TWO HAND WEIGHTS.
IN CONCLUSION , THESE ARE EXCELLENT WEIGHTS THAT I WOULD RECOMMEND TO MY PATIENTS OR FRIENDS. THIS IS A HIGH QUALITY PRODUCT THAT WONT BREAK YOUR PIGGY BANK. I HIGHLY RECOMMEND VERY HIGHLY!. BUY WITHOUT RESERVATION!! A+++
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Reviewed in the United States on March 23, 2025