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Description
Human s-ACE ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell lysis solution: Gently wash adherent cells with pre-cooled PBS, then digest with trypsin, and collect the cells after centrifugation at 1000×g for 5 minutes; suspended cells can be directly collected by centrifugation. Wash the collected cells 3 times with pre-cooled PBS, add 150-200uL PBS for every 1×10^6 cells to resuspend (it is recommended to add protease inhibitors to PBS; if the content is very low, the PBS volume can be appropriately reduced) and break the cells by repeated freezing and thawing or ultrasound. Centrifuge the extract at 2-8℃, 1500×g for 10 minutes, and take the supernatant for detection. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against soluble angiotensin I converting enzyme (s-ACE). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of soluble angiotensin I converting enzyme (s-ACE) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Soluble Angiotensin I Converting Enzyme ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Angiotensin-converting enzyme (ACE) is a core component of the renin-angiotensin system (RAS), which controls blood pressure by regulating fluid volume in the body. It converts the hormone angiotensin I into angiotensin II, an active vasoconstrictor. Thus, ACE indirectly increases blood pressure by causing vasoconstriction. ACE inhibitors are widely used to treat cardiovascular disease. Other lesser-known functions of ACE include the degradation of bradykinin, substance P, and amyloid-beta. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
Soft, Complete Set & Great Value
Color: 01 - Grey, Size: California King, Color: 01 - Grey, Size: California King
I’m really impressed with this comforter set! The material is super soft and comfortable, and it feels great for all seasons, not too heavy but still cozy. I love that it comes with everything you need, which makes it a great value for the price. The gray color is exactly as pictured and gives a clean, modern look to the room. After washing, it held up well with no issues. Overall, a great quality set that I would definitely recommend!
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Holds up beautifully—even after lots of washes
Color: 02 - Olive Green, Size: Queen, Color: 02 - Olive Green, Size: Queen
This is a beautiful bed set and has held up incredibly well over time.
I’ve washed it dozens of times now and figured it was finally time to write a review because it still looks and feels great. No issues with quality, stitching, or softness—it’s held up flawlessly.
The material is soft, warm, and comfortable, and it really pulls the whole look together in our camper bedroom.
Definitely one of those sets that looks good and lasts.
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Reviewed in the United States on April 27, 2026
★★★★★ 5
So comfortable and awesome price.
Color: 01 - Grey, Size: Queen, Color: 01 - Grey, Size: Queen
I really like this bed set. The comforter is soft to touch and is comfortable. It’s weird and funny to say, but my cat really loves it too. The quality is very good! I got it on sale for $30.00… a set that comes with a comforter, fitted sheet, top sheet, 2 regular pillow cases and 2 decorative pillow cases is a win for me. It arrived on time, and came packaged really well. The picture below is showing of how they came in a box from bedsure, the sheets/ pillow cases and comforter come separated in different clear vacuum sealed bags. There are directions on how to open the sealed bags, but I just cut the top of both bags open at the top, and you hear a “pooof” noise, the blanket raised up. I let it air out for a few hours and then put it on my bed and it looked amazing.
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Reviewed in the United States on May 16, 2026
★★★★★ 4
Beautiful Color & Texture – Instantly Upgraded My Bedroom
Color: 07 - Mineral Blue, Size: King, Color: 07 - Mineral Blue, Size: King
I absolutely love this comforter set. The color is rich and calming, and the textured detailing adds such a classy, elegant touch. It looks way more expensive than it actually is and completely transformed my bedroom.
The fabric feels soft and smooth, and it lays nicely on the bed without looking stiff. It gives that clean, polished hotel vibe but still feels cozy and comfortable.
My only small complaint is the size of the comforter. I wish it were a little bigger, especially for fuller coverage on the sides of the bed. If you like that oversized, draped look, you may want to size up if possible. Other than that, it’s perfect.
Overall, I’m very happy with this purchase and would definitely recommend it if you’re looking for something stylish and affordable.
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Reviewed in the United States on February 28, 2026
★★★★★ 5
King size comforter
Color: 11 - Black, Size: California King
I’ve had this black king-size comforter for some time now, and it remains one of my favorite purchases. The comforter is thick, incredibly warm, and ideal for winter. I love the scrunched, textured design, which gives it a modern, stylish look, and the deep black color adds a sleek, elegant touch. It also came with both a set of pillowcases and a fitted sheet, along with a standard flat sheet, offering great versatility. Even now, it adds both style and comfort to my bedroom, and I couldn’t be happier with its quality.
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Reviewed in the United States on May 22, 2026
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