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Description
Mouse SERT ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a serotonin transporter (SERT) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of serotonin transporter (SERT) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Serotonin Transporter ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The serotonin transporter (SERT or 5-HTT), also known as the sodium-dependent serotonin transporter and solute carrier family 6 member 4, is a protein encoded by the SLC6A4 gene. SERT is a monoamine transporter that transports serotonin from the synaptic cleft back into the presynaptic neuron. This transport of serotonin by SERT terminates the effects of serotonin and allows for its recycling in a sodium-dependent manner. This protein is the target of many SSRIs and tricyclic antidepressants. It is a member of the sodium:neurotransmitter synthesizer family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.5 ★★★★★
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Product Reviews
★★★★★ 5
Made my bathroom look beautiful
Color: White, Size: 15.7 Inches
The mounting and the color were excellent. I hung this in my bathroom and thought it was going to be a hard install for me. The hardware that came with this was very self explanatory. The fit was perfect and the quality was great. I would surely buy this item again in the future
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Great wall shelves!
Color: White, Size: 15.7 Inches
Great quality, and firmness. easy to set up. will need a drill to speed up process. White wood finish is really nice, not glossy so perfect for me. width is a little on the shorter side depending on what youre looking to place on them but true to sizing as described
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Reviewed in the United States on May 4, 2026
★★★★★ 4
Floating Shelves
Color: White, Size: 15.7 Inches, Color: White, Size: 15.7 Inches
I purchased a set of white floating shelves for my bedroom a couple months back, and I must say, they have definitely added a touch of modern elegance to the space. The installation process was surprisingly simple and straightforward, making it a breeze to set up the shelves exactly where I wanted them. The package came with all the necessary hardware, including sturdy brackets and screws, ensuring a hassle-free setup experience. I will say that the wall anchors included are not the best quality and I used a third party set.
The sleek and minimalistic design of these floating shelves instantly caught my eye, and I couldn't be happier with how they complement the overall decor of my room. The clean lines and the bright white finish pop with the walls, providing a chic and uncluttered display area for my decorative items, and cherished keepsakes. They truly elevate the aesthetic appeal of any room and create an illusion of more space, which is always a bonus.
However, one aspect that did disappoint me slightly was the visibility of the screws underneath the shelves. While the overall appearance is sleek and sophisticated, this minor flaw hinders the shelves from achieving a truly seamless "floating" effect. I understand that the screws are essential for stability and security, but I wish the manufacturer had found a way to conceal them better, perhaps with a cleverly designed cover or by providing white screw caps.
Despite this minor setback, I still appreciate the functionality and attractiveness of these floating shelves. They have undoubtedly decluttered my living space and allowed me to proudly showcase my favorite items. I have received compliments from friends and family on how the shelves enhance the ambiance of the room.
In conclusion, if you are seeking easy-to-install, stylish white floating shelves to add a contemporary touch to your home, these shelves are an excellent choice. Their seamless installation and sleek appearance make them a delightful addition to any room. Although the visible screws detract slightly from the overall aesthetic, the shelves' overall performance and elegance more than make up for it.
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Reviewed in the United States on July 28, 2023
★★★★★ 5
Good Product
Color: White, Size: 15.7 Inches
Well designed and sturdy. Though not real wood I think they will last. It took me an hour to install the first shelf. That was all on me. I was slow measuring and placing the shelf bracket. I kept dropping the level and doing other crazy things. But the second and third shelves were easy after I learned what I was doing. Even though the screws, etc were provided I used Easy Anchor drywall anchors rated at 50 pounds. Just drill it in the sheet rock then screw the screw through the hole in the bracket until it clicks, (you have to listen). That means the ends of the plastic anchor flips out like a toggle bolt. Continue tightening the screw and that is it. Think I read somewhere that the board wobbles. These were tight as could be and no wobble.
I did learn to only "tighten" just one end of the bracket sort of snug and let it hang. Then raise it to level and mark the other holes with a pencil. Don't put the other screws in until you are sure you can see the plastic anchors' holes though the bracket. Then place and tighten all the screws. The only negative I saw was that they could have sent the same or similar anchors that I have. But they didn't call me. Guess they didn't have my number.
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Reviewed in the United States on March 10, 2026
★★★★★ 5
Easy to Install and Looks Great
Color: White, Size: 15.7 Inches
These floating shelves are a great addition to my daughter room space! They were very easy to install, and the instructions were clear and straightforward. The material feels solid and durable. I also really appreciated that the package included all the necessary hardware and materials, which made the setup even more convenient.
They look great on the wall and serve their purpose perfectly. Highly recommend!
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Reviewed in the United States on April 9, 2026