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Description
Mouse HbC ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed immediately or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a hemoglobin C (HbC) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of hemoglobin C (HbC) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Haemoglobin C ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hemoglobin C (HbC) is an abnormal hemoglobin. It results when a point mutation in the HBB gene replaces glutamic acid with lysine at position 6 of the beta-globin chain of hemoglobin. This mutation can be homologous, occurring on both chromosomes (alleles), or heterologous, affecting only one allele. This mutation can combine with other abnormal hemoglobins to cause severe hemoglobinopathies. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.1 ★★★★★
Based on 988 reviews
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Product Reviews
★★★★★ 3
Wrinkly
Color: 04 - Cherry Pink, Size: Queen
It looks wrinkly, even after washing
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
★★★★★ 5
Beckham Hotel Collection 22 Momme Silk Pillowcase
Color: 04 - Cherry Pink, Size: Queen, Color: 04 - Cherry Pink, Size: Queen
This is a 100% Mulberry silk pillowcase (22 momme, 6A grade) in the Cherry Pink color, Queen size.
Unboxing and Prep
Out of the box, the silk had noticeable deep creases from being folded. I hand washed it and air dried it, but the wrinkles remained. I used a steam iron at approximately 300F with a flour sack towel as a pressing cloth to protect the fabric. It took two passes to get most of the wrinkles out, but the silk held its sheen well throughout the process.
Zipper and Construction
The zipper tracks are hidden behind a fabric flap so you don't feel the teeth. However, the zipper pull is not fully integrated or tucked away; it hangs free at the end of the track. The pull is small enough that it hasn't been an issue or gotten in the way during use, but it's worth noting it isn't completely "invisible."
Fit and Quality
The Cherry Pink is a very light, subtle shade. The 22 momme weight feels substantial and opaque. I paired this with a high-loft Sobel Westex Bellazure Duo (feather and down) pillow. The Queen size fits perfectly and the silk stays smooth and cool.
Final Thoughts
The build quality is solid and the Oeko-TEX certification is a nice touch. To get the best results, you really need to pair this with a high-quality, fluffy pillow to fill out the case properly. On a flat or low-density pillow, it wouldn't look or perform nearly as well. It requires some maintenance with the hand washing and ironing, but the feel is much better than thinner 19 momme options.
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Reviewed in the United States on April 7, 2026
★★★★★ 4
Luxuriously Soft & Cooling, But Needs a Wider Opening
Color: 03 - Champagne, Size: Queen, Color: 03 - Champagne, Size: Queen
I really enjoy sleeping on the Beckham Hotel Collection Silk Pillowcase—it has such a silky, smooth feel that makes a noticeable difference in comfort. It feels wonderful against the skin and hair, and I especially love how it helps keep my head cool throughout the night, which leads to a restful sleep. The color choices are also wonderful and look very rich, adding a nice elegant touch to the bed. It’s also held up nicely in the wash, which is always a plus for something this delicate.
That said, I’m giving it 4 stars because of the zipper opening design. You lose about 4 inches of usable space at the opening, which makes it harder than expected to insert a pillow. Unlike other zippered pillowcases I own, the ends don’t open wide enough, so I had to squeeze my pillow in—and I actually noticed the stitching starting to pull a bit because of it. A wider opening would make a big difference.
Overall, it’s great to sleep on and feels luxurious, but for the price, I do think the design could be improved.
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Reviewed in the United States on April 6, 2026
★★★★★ 5
Best Pillow I’ve bought in a while
Fill Material: Down Alternative, Size: King Size
Very comfy pillow. Holds up better than the last 7 I bought which went flat after a week. It also comes with extra fill which I have not had to use yet but overall very happy!
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Cozy & Comfortable
Fill Material: Down Alternative, Size: Queen/Standard Size
This pillow was a big surprise! Despite the size, it is so soft & comfortable. Being able to add & subtracting filling is wonderful. It's like having a custom pillow just for me. I am a stomach sleeper. I am able to cuddle the pillow & be very comfortable. Plus it recovers it's shape.
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Reviewed in the United States on May 5, 2026